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GSTT1 Rabbit pAb (A1287)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GSTT1 Rabbit pAb (A1287)

Western blot analysis of lysates from HepG2 cells, using GSTT1 Rabbit pAb (A1287) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - GSTT1 Rabbit pAb (A1287)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - GSTT1 Rabbit pAb (A1287)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - GSTT1 Rabbit pAb (A1287)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - GSTT1 Rabbit pAb (A1287)

Immunofluorescence analysis of L929 cells using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGSTT1 Rabbit pAb
Catalog No.A1287
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of GSTT1 (NP_000844.2).
SequenceMGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDAFAQVNPLKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLAWQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDKFLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEAAVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR
Gene ID
Swiss Prot
SynonymsGSTT1
Calculated MW27kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationCytoplasm.
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - GSTT1 Rabbit pAb (A1287)}

    Western blot - GSTT1 Rabbit pAb (A1287)

    Western blot analysis of lysates from HepG2 cells, using GSTT1 Rabbit pAb (A1287) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - GSTT1 Rabbit pAb (A1287)}

    Immunohistochemistry - GSTT1 Rabbit pAb (A1287)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - GSTT1 Rabbit pAb (A1287)}

    Immunohistochemistry - GSTT1 Rabbit pAb (A1287)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - GSTT1 Rabbit pAb (A1287)}

    Immunohistochemistry - GSTT1 Rabbit pAb (A1287)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - GSTT1 Rabbit pAb (A1287)}

    Immunofluorescence - GSTT1 Rabbit pAb (A1287)

    Immunofluorescence analysis of L929 cells using GSTT1 Rabbit pAb (A1287) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A1287? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)