제품 > 항체 > 다중클론항체(pAb)

GTF3C4 Rabbit pAb (A9287)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (A9287) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - GTF3C4 Rabbit pAb (A9287)

Immunofluorescence analysis of C6 cells using GTF3C4 Polyclonal Antibody (A9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (A9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	GTF3C4 Rabbit pAb
Catalog No.	A9287
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable enzyme activator activity. Predicted to contribute to DNA binding activity. Predicted to be involved in 5S class rRNA transcription by RNA polymerase III and tRNA transcription by RNA polymerase III. Located in mitochondrion and nucleoplasm. Part of transcription factor TFIIIC complex.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2).
Sequence	ILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPTTGDAGGREPMEEKLLEIQGKIEAVEMHLTREHMKRVLGEVYLHTWITENTSIPTRGLCNFLMSDEEYDDRTARVLIGHISKKMNKQTFPEHCSLCKEILPFTDRKQAVCSNGHIWLRCFLTYQSCQSLIYRRCLLHDSIARHPAPEDPDWIKRLLQSPCPFCDSPVF
Gene ID
Swiss Prot
Synonyms	KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA
Calculated MW	92kDa
Observed MW	95kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Jurkat, U-251MG, BT-474, HeLa, SW620
Cellular location	Nucleus

Documents

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GTF3C4 Rabbit pAb images

Western blot - GTF3C4 Rabbit pAb (A9287)

Immunofluorescence - GTF3C4 Rabbit pAb (A9287)

Immunofluorescence analysis of C6 cells using GTF3C4 Polyclonal Antibody (A9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - GTF3C4 Rabbit pAb (A9287)

Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (A9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.