Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of various lysates, using HDAC4 Rabbit pAb (A0239) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | HDAC4 Rabbit pAb |
|---|---|
| Catalog No. | A0239 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 530-630 of human HDAC4 (NP_006028.2). |
|---|---|
| Sequence | REHQALLDEPYLDRLPGQKEAHAQAGVQVKQEPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A; NEDCHF; NEDCHID; HDAC4 |
| Calculated MW | 119kDa |
| Observed MW | 140kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa |
| Cellular location | Cytoplasm, Nucleus |
| Customer validation | WB(Mus musculus, Homo sapiens) IF(Mus musculus) ELISA(Homo sapiens) IHC(Mus musculus) IF(Mus musculus) IP(Gallus gallus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A0239? Please let us know so that we can cite the reference in this datasheet.
