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HIBADH Rabbit pAb (A21349)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HIBADH Rabbit pAb (A21349)

Western blot analysis of various lysates, using HIBADH Rabbit pAb (A21349) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HIBADH Rabbit pAb (A21349)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HIBADH Rabbit pAb (A21349) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - HIBADH Rabbit pAb (A21349)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using HIBADH Rabbit pAb (A21349) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - HIBADH Rabbit pAb (A21349)

Immunofluorescence analysis of C6 cells using HIBADH Rabbit pAb (A21349) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HIBADH Rabbit pAb (A21349)

Immunofluorescence analysis of L929 cells using HIBADH Rabbit pAb (A21349) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HIBADH Rabbit pAb (A21349)

Immunofluorescence analysis of U-2 OS cells using HIBADH Rabbit pAb (A21349) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHIBADH Rabbit pAb
Catalog No.A21349
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human HIBADH (NP_689953.1).
SequenceASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAEKADRIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVSKELAKEVEKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQFLREEETF
Gene ID
Swiss Prot
SynonymsNS5ATP1; HIBADH
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, 293T, Mouse brain, Mouse heart, Rat heart
Cellular locationMitochondrion.

Documents

Certificate of Compliance

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ABclonal:Western blot - HIBADH Rabbit pAb (A21349)}

Western blot - HIBADH Rabbit pAb (A21349)

Western blot analysis of various lysates, using HIBADH Rabbit pAb (A21349) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - HIBADH Rabbit pAb (A21349)}

Immunohistochemistry - HIBADH Rabbit pAb (A21349)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HIBADH Rabbit pAb (A21349) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - HIBADH Rabbit pAb (A21349)}

Immunohistochemistry - HIBADH Rabbit pAb (A21349)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using HIBADH Rabbit pAb (A21349) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - HIBADH Rabbit pAb (A21349)}

Immunofluorescence - HIBADH Rabbit pAb (A21349)

Immunofluorescence analysis of C6 cells using HIBADH Rabbit pAb (A21349) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HIBADH Rabbit pAb (A21349)}

Immunofluorescence - HIBADH Rabbit pAb (A21349)

Immunofluorescence analysis of L929 cells using HIBADH Rabbit pAb (A21349) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HIBADH Rabbit pAb (A21349)}

Immunofluorescence - HIBADH Rabbit pAb (A21349)

Immunofluorescence analysis of U-2 OS cells using HIBADH Rabbit pAb (A21349) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (25)