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HIF3A Rabbit pAb (A20020)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HIF3A Rabbit pAb (A20020)

Western blot analysis of 293F-HIF3A-FlagN, using HIF3A Rabbit pAb (A20020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - HIF3A Rabbit pAb (A20020)

Immunofluorescence analysis of C6 cells using HIF3A Rabbit pAb (A20020) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameHIF3A Rabbit pAb
Catalog No.A20020
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human HIF3A (NP_690008.2).
SequenceMALGLQRARSTTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLCAAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIF
Gene ID
Swiss Prot
SynonymsIPAS; MOP7; PASD7; HIF-3A; bHLHe17; HIF3-alpha-1
Calculated MW72kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293F-HIF3A-FlagN
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus,Oryctolagus cuniculus)

Documents

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ABclonal:Western blot - HIF3A Rabbit pAb (A20020)}

Western blot - HIF3A Rabbit pAb (A20020)

Western blot analysis of 293F-HIF3A-FlagN, using HIF3A Rabbit pAb (A20020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - HIF3A Rabbit pAb (A20020)}

Immunofluorescence - HIF3A Rabbit pAb (A20020)

Immunofluorescence analysis of C6 cells using HIF3A Rabbit pAb (A20020) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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