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HMGCR Rabbit pAb (A1633)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of lysates from Rat brain, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of lysates from Mouse spleen, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry analysis of paraffin-embedded Human small intestine using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of C6 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of NIH-3T3 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of U-2 OS cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of NIH/3T3 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of PC-12 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of U2OS cells using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHMGCR Rabbit pAb
Catalog No.A1633
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 731-830 of human HMGCR (NP_000850.1).
SequenceVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTA
Gene ID
Swiss Prot
SynonymsLDLCQ3; HMGCR
Calculated MW97kDa
Observed MW97kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen, Rat brain
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

qPCR(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - HMGCR Rabbit pAb (A1633)}

Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of lysates from Rat brain, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - HMGCR Rabbit pAb (A1633)}

Western blot - HMGCR Rabbit pAb (A1633)

Western blot analysis of lysates from Mouse spleen, using HMGCR Rabbit pAb (A1633) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633)}

Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry analysis of paraffin-embedded Human small intestine using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - HMGCR Rabbit pAb (A1633)}

Immunohistochemistry - HMGCR Rabbit pAb (A1633)

Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)}

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of C6 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)}

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of NIH-3T3 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)}

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of U-2 OS cells using HMGCR Rabbit pAb (A1633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)}

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of NIH/3T3 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)}

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of PC-12 cells using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HMGCR Rabbit pAb (A1633)}

Immunofluorescence - HMGCR Rabbit pAb (A1633)

Immunofluorescence analysis of U2OS cells using HMGCR Rabbit pAb (A1633) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)