제품 > 항체 > 다중클론항체(pAb)

HNRNPA0 Rabbit pAb (A6029)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HNRNPA0 Rabbit pAb (A6029)

Western blot analysis of various lysates using HNRNPA0 Rabbit pAb (A6029) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - HNRNPA0 Rabbit pAb (A6029)

Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg HNRNPA0 antibody (A6029). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (A6029) at a dilution of 1:1000.

Review
ABclonal: review for HNRNPA0 Rabbit pAb(A6029)

Overview

Product nameHNRNPA0 Rabbit pAb
Catalog No.A6029
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).
SequenceMENSQLCKLFIGGLNVQTSESGLRGHFEAFGTLTDCVVVVNPQTKRSRCFGFVTYSNVEEADAAMAASPHAVDGNTVELKRAVSREDSARPGAHAKVKKLFVGGLKGDVAEGDLIEHFSQFGTVEKAEIIADKQSGKKRGFGFVYFQNHDAADKAAVVKFHPIQGHRVEVKKAVPKEDIY
Gene ID
Swiss Prot
SynonymsHNRPA0; HNRNPA0
Calculated MW31kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesMCF-7, BT-474, SKOV3, 293T, HeLa, Mouse brain, Mouse spleen, Rat brain
Cellular locationNucleus
Customer validation

(293T)

Documents

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ABclonal:Western blot - HNRNPA0 Rabbit pAb (A6029)}

Western blot - HNRNPA0 Rabbit pAb (A6029)

Western blot analysis of various lysates using HNRNPA0 Rabbit pAb (A6029) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - HNRNPA0 Rabbit pAb (A6029)}

Immunoprecipitation - HNRNPA0 Rabbit pAb (A6029)

Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg HNRNPA0 antibody (A6029). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (A6029) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)