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HNRNPD Rabbit mAb (A23281)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HNRNPD Rabbit mAb (A23281)

Western blot analysis of various lysates, using HNRNPD Rabbit mAb (A23281) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Human liver using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Rat brain using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of A-431 cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of C6 cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of HeLa cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of NIH/3T3 cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of U2OS cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of U-251MG cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHNRNPD Rabbit mAb
Catalog No.A23281
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59955
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPD. (NP_112738.1).
SequenceMSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRAR
Gene ID
Swiss Prot
SynonymsP37; AUF1; AUF1A; HNRPD; hnRNPD0; HNRNPD
Calculated MW38kDa
Observed MW37-48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, NIH/3T3, C6
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - HNRNPD Rabbit mAb (A23281)}

Western blot - HNRNPD Rabbit mAb (A23281)

Western blot analysis of various lysates, using HNRNPD Rabbit mAb (A23281) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)}

Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Human liver using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)}

Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)}

Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - HNRNPD Rabbit mAb (A23281)}

Immunohistochemistry - HNRNPD Rabbit mAb (A23281)

Immunohistochemistry analysis of paraffin-embedded Rat brain using HNRNPD Rabbit mAb (A23281) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)}

Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of A-431 cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)}

Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of C6 cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)}

Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of HeLa cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)}

Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of NIH/3T3 cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)}

Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of U2OS cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HNRNPD Rabbit mAb (A23281)}

Immunofluorescence - HNRNPD Rabbit mAb (A23281)

Immunofluorescence analysis of U-251MG cells using HNRNPD Rabbit mAb (A23281) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)