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HSD17B1 Rabbit pAb (A10839)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HSD17B1 Rabbit pAb (A10839)

Western blot analysis of various lysates using HSD17B1 Rabbit pAb (A10839) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - HSD17B1 Rabbit pAb (A10839)

Immunohistochemistry analysis of paraffin-embedded human placenta using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HSD17B1 Rabbit pAb (A10839)

Immunohistochemistry analysis of paraffin-embedded mouse heart using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HSD17B1 Rabbit pAb (A10839)

Immunofluorescence analysis of C6 cells using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSD17B1 Rabbit pAb (A10839)

Immunofluorescence analysis of HeLa cells using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHSD17B1 Rabbit pAb
Catalog No.A10839
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human HSD17B1 (NP_000404.2).
SequenceMARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVTEGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKFALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ
Gene ID
Swiss Prot
SynonymsE2DH; HSD17; EDHB17; EDH17B2; SDR28C1; 17-beta-HSD; 20-alpha-HSD; HSD17B1
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, Rat ovary
Cellular locationCytoplasm
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Rattus norvegicus)

WB(Rattus norvegicus)

IF(Rattus norvegicus)

IF(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - HSD17B1 Rabbit pAb (A10839)}

    Western blot - HSD17B1 Rabbit pAb (A10839)

    Western blot analysis of various lysates using HSD17B1 Rabbit pAb (A10839) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - HSD17B1 Rabbit pAb (A10839)}

    Immunohistochemistry - HSD17B1 Rabbit pAb (A10839)

    Immunohistochemistry analysis of paraffin-embedded human placenta using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - HSD17B1 Rabbit pAb (A10839)}

    Immunohistochemistry - HSD17B1 Rabbit pAb (A10839)

    Immunohistochemistry analysis of paraffin-embedded mouse heart using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - HSD17B1 Rabbit pAb (A10839)}

    Immunofluorescence - HSD17B1 Rabbit pAb (A10839)

    Immunofluorescence analysis of C6 cells using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - HSD17B1 Rabbit pAb (A10839)}

    Immunofluorescence - HSD17B1 Rabbit pAb (A10839)

    Immunofluorescence analysis of HeLa cells using HSD17B1 Rabbit pAb (A10839) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A10839? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)