Reviews (2)Publications (19) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using HSP70 Rabbit pAb (A0284) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of lysates from Mouse brain using HSP70 Rabbit pAb (A0284) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of A549 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using HSP70 Rabbit pAb(A0284) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | HSP70 Rabbit pAb |
|---|---|
| Catalog No. | A0284 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3). |
|---|---|
| Sequence | KITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HSP70; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103 |
| Calculated MW | 70kDa |
| Observed MW | 70kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | Mouse brain, 293T, HeLa, A549 |
| Cellular location | blood microparticle, centriole, centrosome, cytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, extracellular region, focal adhesion, mitochondrion, nuclear speck, nucleoplasm, nucleus, perinuclear region of cytoplasm, plasma membrane. |
| Customer validation | (HK2, HeLa) WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus) IHC(Mus musculus) IF(Homo sapiens) IF(Homo sapiens,Mus musculus) |
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_IHC_01.jpg?t=1718615971 "ABclonal:Immunohistochemistry - HSP70 Rabbit pAb (A0284)")
_IHC_02.jpg?t=1718615971 "ABclonal:Immunohistochemistry - HSP70 Rabbit pAb (A0284)")
