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Human Parkin Rabbit pAb (A0968)

Publications (58) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Human Parkin Rabbit pAb (A0968)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Parkin using Parkin Rabbit pAb (A0968) at 1:1000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Human Parkin Rabbit pAb (A0968)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Parkin Rabbit pAb (A0968) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Human Parkin Rabbit pAb (A0968)

Immunofluorescence analysis of paraffin-embedded mouse brain using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Human Parkin Rabbit pAb (A0968)

Immunofluorescence analysis of paraffin-embedded rat brain using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameHuman Parkin Rabbit pAb
Catalog No.A0968
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human Parkin (NP_004553.2).
SequenceNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHD
Gene ID
Swiss Prot
SynonymsPDJ; AR-JP; LPRS2; PARK2; Parkin
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293F transfected with Parkin
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, cytosol.
Customer validation

WB(Homo sapiens, Gallus gallus, Rattus norvegicus, Mus musculus, Dianella ensifolia, Mus musculus, Homo sapiens, Other, Sus scrofa)

IHC(Mus musculus)

IF(Mus musculus, Homo sapiens)

IF(Homo sapiens, Mus musculus, Homo sapiens)

IP(Homo sapiens)

IP(Mus musculus)

WB(Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Human Parkin Rabbit pAb (A0968)}

Western blot - Human Parkin Rabbit pAb (A0968)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Parkin using Parkin Rabbit pAb (A0968) at 1:1000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Human Parkin Rabbit pAb (A0968)}

Immunohistochemistry - Human Parkin Rabbit pAb (A0968)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Parkin Rabbit pAb (A0968) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Human Parkin Rabbit pAb (A0968)}

Immunofluorescence - Human Parkin Rabbit pAb (A0968)

Immunofluorescence analysis of paraffin-embedded mouse brain using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Human Parkin Rabbit pAb (A0968)}

Immunofluorescence - Human Parkin Rabbit pAb (A0968)

Immunofluorescence analysis of paraffin-embedded rat brain using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0968? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

ELISA 키트 (1)

Secondary Antibodies (26)