제품 > 항체 > 다중클론항체(pAb)

IBSP Rabbit pAb (A16220)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IBSP Rabbit pAb (A16220)

Western blot analysis of various lysates, using IBSP Rabbit pAb (A16220) at 1:2500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameIBSP Rabbit pAb
Catalog No.A16220
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human IBSP (NP_004958.2).
SequenceMKTALILLSILGMACAFSMKNLHRRVKIEDSEENGVFKYRPRYYLYKHAYFYPHLKRFPVQGSSDSSEENGDDSSEEEEEEEETSNEGENNEESNEDEDS
Gene ID
Swiss Prot
SynonymsBSP; BNSP; SP-II; BSP-II; IBSP
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU2OS, U-138MG, Rat lung
Cellular locationSecreted.
Customer validation

WB(Canis lupus familiaris, Mus musculus, Bos taurus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - IBSP Rabbit pAb (A16220)}

Western blot - IBSP Rabbit pAb (A16220)

Western blot analysis of various lysates, using IBSP Rabbit pAb (A16220) at 1:2500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16220? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)