제품 > 항체 > 단일클론항체(mAb)

ICAM2 / CD102 Rabbit mAb (A20870)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of extracts of various cell lines, using ICAM2/CD102 antibody (A20870) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	ICAM2 / CD102 Rabbit mAb
Catalog No.	A20870
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2802

Background

Predicted to enable integrin binding activity. Predicted to be involved in cell adhesion. Located in microvillus; plasma membrane; and uropod. Is integral component of plasma membrane. Is expressed in central nervous system; extraembryonic component; female reproductive system; retina; and vascular element. Orthologous to human ICAM2 (intercellular adhesion molecule 2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of mouse ICAM2 / CD102 (P35330).
Sequence	MSSFACWSLSLLILFYSPGSGEKAFEVYIWSEKQIVEATESWKINCSTNCAAPDMGGLETPTNKIMLEEHPQGKWKQFLVSNVSKDTVFFCHFTCSGKQHSESLNIRVYQPPAQVTLKLQPPRVFVGEDFTIECTVSPVQPLERLTLSLLRGRETLKNQTFGGAETVPQEATATFNSTALKKDGLNFSCQAELDLRPHGGYIIRSISEYQILEVYEPMQDNQMVIIIVVVSILLFLFVTS
Gene ID
Swiss Prot
Synonyms	CD102; Ly-60; Icam-2
Calculated MW	31kDa
Observed MW	45kDa/55kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse lung, Rat lung
Cellular location	microvillus, plasma membrane