제품 > 항체 > 다중클론항체(pAb)

IGF2 Rabbit pAb (A2086)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HepG2 cells, using IGF2 Rabbit pAb (A2086) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IGF2 Rabbit pAb (A2086)

Immunohistochemistry analysis of paraffin-embedded Human placenta using IGF2 Rabbit pAb (A2086) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - IGF2 Rabbit pAb (A2086)

Immunofluorescence analysis of HepG2 cells using IGF2 Rabbit pAb (A2086) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	IGF2 Rabbit pAb
Catalog No.	A2086
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human IGF2 (NP_000603.1).
Sequence	AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPAKSERDVSTPPTVLPDNFPRYPVGKFFQYDTWKQSTQRLRRGLPALLRARRGHVLAKELEAFREAKRHRPLIALPTQDPAHGGAPPEMASNRK
Gene ID
Swiss Prot
Synonyms	GRDF; SRS3; IGF-II; PP9974; C11orf43; IGF2
Calculated MW	20kDa
Observed MW	20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2
Cellular location	Secreted.
Customer validation	WB(Rattus norvegicus, Gallus gallus, Mus musculus, Homo sapiens) IHC(Cervus nippon, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

IGF2 Rabbit pAb images

Western blot - IGF2 Rabbit pAb (A2086)

Immunohistochemistry - IGF2 Rabbit pAb (A2086)

Immunofluorescence - IGF2 Rabbit pAb (A2086)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

Life Sciences

Application:

IF:

5.037

Species:

Rattus norvegicus

PMID:

26522050

Title:

The proteins interacting with C-terminal of μ receptor are identified by bacterial two-hybrid system from brain cDNA library in morphine-dependent rats

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

International Journal of Molecular Sciences

Application:

IF:

5.923

Species:

Gallus gallus

PMID:

32121275

Title:

miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

Cell Rep

Application:

IF:

9.423

Species:

Mus musculus

PMID:

33567274

Title:

Prenatal correction of IGF2 to rescue the growth phenotypes in mouse models of Beckwith-Wiedemann and Silver-Russell syndromes

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

Cell Mol Gastroenterol Hepatol

Application:

IF:

9.225

Species:

Mus musculus

PMID:

34438112

Title:

Activation of Proneuronal Transcription Factor Ascl1 in Maternal Liver Ensures a Healthy Pregnancy

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

Cell regeneration (London, England)

Application:

IHC

IF:

Species:

Cervus nippon

PMID:

36542206

Title:

Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

Journal of orthopaedic surgery and research

Application:

IF:

2.67

Species:

Homo sapiens

PMID:

37415192

Title:

Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis - PMC

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

BMC cancer

Application:

IF:

3.8

Species:

Mus musculus

PMID:

38644473

Title:

PROTAC EZH2 degrader-1 overcomes the resistance of podophyllotoxin derivatives in refractory small cell lung cancer with leptomeningeal metastasis - PMC

References for IGF2 Rabbit pAb (A2086)

Product:

IGF2 Rabbit pAb

Journal:

Diagnostic pathology

Application:

IHC

IF:

2.6

Species:

Homo sapiens

PMID:

38802933

Title:

Diagnostic and prognostic assessments of adrenocortical carcinomas by pathological features, immunohistochemical markers and reticular histochemistry staining - PMC