제품 > 항체 > 다중클론항체(pAb)

IGFBP5 Rabbit pAb (A12451)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)

Western blot analysis of lysates from Mouse kidney, using IGFBP5 Rabbit pAb (A12451) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)

Western blot analysis of lysates from Mouse uterus, using IGFBP5 Rabbit pAb (A12451) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)

Western blot analysis of lysates from Rat kidney, using IGFBP5 Rabbit pAb (A12451) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)

Western blot analysis of lysates from 293T cells, using IGFBP5 Rabbit pAb (A12451) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - IGFBP5 Rabbit pAb (A12451)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - IGFBP5 Rabbit pAb (A12451)

Immunohistochemistry analysis of paraffin-embedded Rat brain using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

Immunofluorescence analysis of HeLa cells using IGFBP5 Rabbit pAb (A12451).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

Immunofluorescence analysis of NIH/3T3 cells using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

Immunofluorescence analysis of PC-12 cells using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

Immunofluorescence analysis of U2OS cells using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIGFBP5 Rabbit pAb
Catalog No.A12451
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables insulin-like growth factor I binding activity. Involved in several processes, including cellular response to cAMP; regulation of smooth muscle cell migration; and regulation of smooth muscle cell proliferation. Part of insulin-like growth factor ternary complex. Biomarker of pulmonary fibrosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1).
SequenceGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVG
Gene ID
Swiss Prot
SynonymsIBP5; IGFBP5
Calculated MW31kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse kidney
Cellular locationSecreted.
Customer validation

IHC(Mus musculus)

WB(Mus musculus)

WB(Homo sapiens)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)}

    Western blot - IGFBP5 Rabbit pAb (A12451)

    Western blot analysis of lysates from Mouse kidney, using IGFBP5 Rabbit pAb (A12451) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)}

    Western blot - IGFBP5 Rabbit pAb (A12451)

    Western blot analysis of lysates from Mouse uterus, using IGFBP5 Rabbit pAb (A12451) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)}

    Western blot - IGFBP5 Rabbit pAb (A12451)

    Western blot analysis of lysates from Rat kidney, using IGFBP5 Rabbit pAb (A12451) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - IGFBP5 Rabbit pAb (A12451)}

    Western blot - IGFBP5 Rabbit pAb (A12451)

    Western blot analysis of lysates from 293T cells, using IGFBP5 Rabbit pAb (A12451) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - IGFBP5 Rabbit pAb (A12451)}

    Immunohistochemistry - IGFBP5 Rabbit pAb (A12451)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - IGFBP5 Rabbit pAb (A12451)}

    Immunohistochemistry - IGFBP5 Rabbit pAb (A12451)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)}

    Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

    Immunofluorescence analysis of HeLa cells using IGFBP5 Rabbit pAb (A12451).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
    ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)}

    Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

    Immunofluorescence analysis of NIH/3T3 cells using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)}

    Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

    Immunofluorescence analysis of PC-12 cells using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IGFBP5 Rabbit pAb (A12451)}

    Immunofluorescence - IGFBP5 Rabbit pAb (A12451)

    Immunofluorescence analysis of U2OS cells using IGFBP5 Rabbit pAb (A12451) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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