제품 > 항체 > 다중클론항체(pAb)

IGFBPL1 Rabbit pAb (A16635)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IGFBPL1 Rabbit pAb (A16635)

Western blot analysis of various lysates using IGFBPL1 Rabbit pAb (A16635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.

Overview

Product nameIGFBPL1 Rabbit pAb
Catalog No.A16635
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable insulin-like growth factor binding activity. Involved in cellular response to tumor cell. Located in extracellular space.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 150-250 of human IGFBPL1 (NP_001007564.1).
SequencePCEFAPVVVVPPRSVHNVTGAQVGLSCEVRAVPTPVITWRKVTKSPEGTQALEELPGDHVNIAVQVRGGPSDHEATAWILINPLRKEDEGVYQCHAANMVG
Gene ID
Swiss Prot
SynonymsIGFBPRP4; IGFBP-RP4; bA113O24.1; IGFBPL1
Calculated MW29kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y, U-87MG
Cellular locationextracellular space

Documents

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    ABclonal:Western blot - IGFBPL1 Rabbit pAb (A16635)}

    Western blot - IGFBPL1 Rabbit pAb (A16635)

    Western blot analysis of various lysates using IGFBPL1 Rabbit pAb (A16635) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 300s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)