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IHH Rabbit pAb (A6626)

Review (1)Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IHH Rabbit pAb (A6626)

Western blot analysis of extracts of various cell lines, using IHH antibody (A6626) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review
ABclonal: review for IHH Rabbit pAb(A6626)

Overview

Product nameIHH Rabbit pAb
Catalog No.A6626
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 202-411 of human IHH (NP_002172.2).
SequenceGCFPAGAQVRLESGARVALSAVRPGDRVLAMGEDGSPTFSDVLIFLDREPHRLRAFQVIETQDPPRRLALTPAHLLFTADNHTEPAARFRATFASHVQPGQYVLVAGVPGLQPARVAAVSTHVALGAYAPLTKHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFHSLAWGSWTPGEGVHWYPQLLYRLGRLLLEEGSFHPLGMSGAGS
Gene ID
Swiss Prot
SynonymsBDA1; HHG2
Calculated MW45kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, 293T, HepG2, Mouse large intestine, Mouse kidney, Mouse stomach, Rat large intestine, Rat kidney, Rat stomach
Cellular locationCell membrane, Extracellular side, Lipid-anchor, Secreted, extracellular space
Customer validation

(Mouse liver)

WB(Mus musculus, Homo sapiens, Gallus gallus)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - IHH Rabbit pAb (A6626)}

Western blot - IHH Rabbit pAb (A6626)

Western blot analysis of extracts of various cell lines, using IHH antibody (A6626) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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재조합 단백질 (1)

Secondary Antibodies (26)