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IKBKAP Rabbit pAb (A10127)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IKBKAP Rabbit pAb (A10127)

Western blot analysis of various lysates using IKBKAP Rabbit pAb (A10127) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IKBKAP Rabbit pAb (A10127)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using IKBKAP Rabbit pAb (A10127) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IKBKAP Rabbit pAb (A10127)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using IKBKAP Rabbit pAb (A10127) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IKBKAP Rabbit pAb (A10127)

Immunohistochemistry analysis of paraffin-embedded rat stomach using IKBKAP Rabbit pAb (A10127) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameIKBKAP Rabbit pAb
Catalog No.A10127
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1093-1332 of human IKBKAP (NP_003631.2).
SequenceLRLVYKYNRLDIIETNVKPSILEAQKNYMAFLDSQTATFSRHKKRLLVVRELKEQAQQAGLDDEVPHGQESDLFSETSSVVSGSEMSGKYSHSNSRISARSSKNRRKAERKKHSLKEGSPLEDLALLEALSEVVQNTENLKDEVYHILKVLFLFEFDEQGRELQKAFEDTLQLMERSLPEIWTLTYQQNSATPVLGPNSTANSIMASYQQQKTSVPVLDAELFIPPKINRRTQWKLSLLD
Gene ID
Swiss Prot
SynonymsFD; DYS; IKAP; IKI3; TOT1; IKBKAP
Calculated MW150kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HepG2, U-251MG, HL-60, Mouse liver, Rat liver
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - IKBKAP Rabbit pAb (A10127)}

    Western blot - IKBKAP Rabbit pAb (A10127)

    Western blot analysis of various lysates using IKBKAP Rabbit pAb (A10127) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - IKBKAP Rabbit pAb (A10127)}

    Immunohistochemistry - IKBKAP Rabbit pAb (A10127)

    Immunohistochemistry analysis of paraffin-embedded human liver cancer using IKBKAP Rabbit pAb (A10127) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - IKBKAP Rabbit pAb (A10127)}

    Immunohistochemistry - IKBKAP Rabbit pAb (A10127)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using IKBKAP Rabbit pAb (A10127) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - IKBKAP Rabbit pAb (A10127)}

    Immunohistochemistry - IKBKAP Rabbit pAb (A10127)

    Immunohistochemistry analysis of paraffin-embedded rat stomach using IKBKAP Rabbit pAb (A10127) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)