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IL13RA1 Rabbit mAb (A3524)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - IL13RA1 Rabbit mAb (A3524)

Western blot analysis of various lysates using IL13RA1 Rabbit mAb (A3524) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IL13RA1 Rabbit mAb (A3524)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using IL13RA1 Rabbit mAb (A3524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameIL13RA1 Rabbit mAb
Catalog No.A3524
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2027
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL13RA1 (P78552).
SequenceMEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGS
Gene ID
Swiss Prot
SynonymsNR4; CT19; CD213A1; IL-13Ra; IL13RA1
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Hep G2, A549
Cellular locationexternal side of plasma membrane, plasma membrane

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ABclonal:Western blot - IL13RA1 Rabbit mAb (A3524)}

Western blot - IL13RA1 Rabbit mAb (A3524)

Western blot analysis of various lysates using IL13RA1 Rabbit mAb (A3524) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - IL13RA1 Rabbit mAb (A3524)}

Immunohistochemistry - IL13RA1 Rabbit mAb (A3524)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using IL13RA1 Rabbit mAb (A3524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)