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IL27RA Rabbit pAb (A8176)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IL27RA Rabbit pAb (A8176)

Western blot analysis of various lysates using IL27RA Rabbit pAb (A8176) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IL27RA Rabbit pAb (A8176)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IL27RA Rabbit pAb (A8176) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IL27RA Rabbit pAb (A8176)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using IL27RA Rabbit pAb (A8176) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameIL27RA Rabbit pAb
Catalog No.A8176
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 33-283 of human IL27RA (NP_004834.1).
SequenceQGSAGPLQCYGVGPLGDLNCSWEPLGDLGAPSELHLQSQKYRSNKTQTVAVAAGRSWVAIPREQLTMSDKLLVWGTKAGQPLWPPVFVNLETQMKPNAPRLGPDVDFSEDDPLEATVHWAPPTWPSHKVLICQFHYRRCQEAAWTLLEPELKTIPLTPVEIQDLELATGYKVYGRCRMEKEEDLWGEWSPILSFQTPPSAPKDVWVSGNLCGTPGGEEPLLLWKAPGPCVQVSYKVWFWVGGRELSPEGIT
Gene ID
Swiss Prot
SynonymsCRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1; IL27RA
Calculated MW69kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7, Mouse liver, Rat liver
Cellular locationMembrane, Single-pass type I membrane protein.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - IL27RA Rabbit pAb (A8176)}

Western blot - IL27RA Rabbit pAb (A8176)

Western blot analysis of various lysates using IL27RA Rabbit pAb (A8176) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - IL27RA Rabbit pAb (A8176)}

Immunohistochemistry - IL27RA Rabbit pAb (A8176)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IL27RA Rabbit pAb (A8176) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IL27RA Rabbit pAb (A8176)}

Immunohistochemistry - IL27RA Rabbit pAb (A8176)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using IL27RA Rabbit pAb (A8176) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)