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IL2 Rabbit mAb (A22200)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - IL2 Rabbit mAb (A22200)

Western blot analysis of Recombinant Mouse IL-2 Protein (RP01384), using IL2 Rabbit mAb (A22200) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.

Overview

Product nameIL2 Rabbit mAb
Catalog No.A22200
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55614
Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-169 of mouse IL2 (NP_032392.1).
SequenceAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ
Gene ID
Swiss Prot
SynonymsIl-2; IL2
Calculated MW19kDa
Observed MW22kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRecombinant Mouse IL-2 protein
Cellular locationextracellular space.

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    ABclonal:Western blot - IL2 Rabbit mAb (A22200)}

    Western blot - IL2 Rabbit mAb (A22200)

    Western blot analysis of Recombinant Mouse IL-2 Protein (RP01384), using IL2 Rabbit mAb (A22200) at1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 10ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 0.2s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    ELISA 키트 (2)

    재조합 단백질 (1)

    항체 (1)

    Secondary Antibodies (26)