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IL6ST Rabbit pAb (A18036)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IL6ST Rabbit pAb (A18036)

Western blot analysis of various lysates using IL6ST Rabbit pAb (A18036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - IL6ST Rabbit pAb (A18036)

Immunofluorescence analysis of NIH/3T3 cells using IL6ST Rabbit pAb (A18036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IL6ST Rabbit pAb (A18036)

Immunofluorescence analysis of U2OS cells using IL6ST Rabbit pAb (A18036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIL6ST Rabbit pAb
Catalog No.A18036
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 751-918 of human IL6ST (NP_002175.2).
SequenceQNTSSTVQYSTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDGGDGILPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSGQMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMPQ
Gene ID
Swiss Prot
SynonymsCD130; GP130; HIES4; IMD94; STWS2; CDW130; HIES4A; HIES4B; IL-6RB; sGP130; IL6ST
Calculated MW104kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver, Rat liver
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein
Customer validation

IF(Homo sapiens)

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - IL6ST Rabbit pAb (A18036)}

Western blot - IL6ST Rabbit pAb (A18036)

Western blot analysis of various lysates using IL6ST Rabbit pAb (A18036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - IL6ST Rabbit pAb (A18036)}

Immunofluorescence - IL6ST Rabbit pAb (A18036)

Immunofluorescence analysis of NIH/3T3 cells using IL6ST Rabbit pAb (A18036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL6ST Rabbit pAb (A18036)}

Immunofluorescence - IL6ST Rabbit pAb (A18036)

Immunofluorescence analysis of U2OS cells using IL6ST Rabbit pAb (A18036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)