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Insulin Rabbit mAb (A19066)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Insulin Rabbit mAb (A19066)

Western blot analysis of various lysates using Insulin Rabbit mAb (A19066) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Insulin Rabbit mAb (A19066)

Immunohistochemistry analysis of paraffin-embedded Rat pancreatic islet tissue using Insulin Rabbit mAb (A19066) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Insulin Rabbit mAb (A19066)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreatic islet tissue using Insulin Rabbit mAb (A19066) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Insulin Rabbit mAb (A19066)

Confocal imaging of paraffin-embedded Human pancreas using Insulin Rabbit mAb (A19066, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameInsulin Rabbit mAb
Catalog No.A19066
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0209
This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Insulin (P01308).
SequenceMALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSIC
Gene ID
Swiss Prot
SynonymsIDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10; Insulin
Calculated MW12kDa
Observed MW12kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:500 - 1:500
  • IF/ICC 1:100 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse pancreas, Rat pancreas
Cellular locationSecreted.
Customer validation

IF(Mus musculus, Gallus gallus)

IHC(Mus musculus)

WB(Homo sapiens, Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Insulin Rabbit mAb (A19066)}

Western blot - Insulin Rabbit mAb (A19066)

Western blot analysis of various lysates using Insulin Rabbit mAb (A19066) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - Insulin Rabbit mAb (A19066)}

Immunohistochemistry - Insulin Rabbit mAb (A19066)

Immunohistochemistry analysis of paraffin-embedded Rat pancreatic islet tissue using Insulin Rabbit mAb (A19066) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - Insulin Rabbit mAb (A19066)}

Immunohistochemistry - Insulin Rabbit mAb (A19066)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreatic islet tissue using Insulin Rabbit mAb (A19066) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - Insulin Rabbit mAb (A19066)}

Immunofluorescence - Insulin Rabbit mAb (A19066)

Confocal imaging of paraffin-embedded Human pancreas using Insulin Rabbit mAb (A19066, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19066? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

ELISA 키트 (1)

Secondary Antibodies (26)