제품 > 항체 > 다중클론항체(pAb)

KDM3B Rabbit pAb (A2312)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - KDM3B Rabbit pAb (A2312)

Western blot analysis of lysates from Hela cells, using KDM3B Rabbit pAb (A2312) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - KDM3B Rabbit pAb (A2312)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using KDM3B Rabbit pAb (A2312) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameKDM3B Rabbit pAb
Catalog No.A2312
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable chromatin DNA binding activity; histone H3-methyl-lysine-9 demethylase activity; and transcription coregulator activity. Predicted to be involved in histone H3-K9 demethylation and regulation of transcription by RNA polymerase II. Located in nucleoplasm. Biomarker of acute lymphoblastic leukemia; breast cancer; colorectal cancer; and lung non-small cell carcinoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human KDM3B (NP_057688.2).
SequenceGAENKEAGKTLEQVGQGIVASAAVVTTASSTPNTVRISDTGLAAGTVPEKQKGSRSQASGENSRNSILASSGFGAPLPSSSQPLTFGSGRSQSNGVLATENKPLGFSFGCSSAQEAQKDTDLSKNLFFQCMSQTLPTSNYFTTVSESLADDSSSRDSFKQSLESLSSGLCKGRSVLGTDTKPGSKAGSSVDRKVPAESMPT
Gene ID
Swiss Prot
Synonyms5qNCA; DIJOS; NET22; C5orf7; JMJD1B; KDM3B
Calculated MW192kDa
Observed MW225kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationNucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - KDM3B Rabbit pAb (A2312)}

Western blot - KDM3B Rabbit pAb (A2312)

Western blot analysis of lysates from Hela cells, using KDM3B Rabbit pAb (A2312) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - KDM3B Rabbit pAb (A2312)}

Immunohistochemistry - KDM3B Rabbit pAb (A2312)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using KDM3B Rabbit pAb (A2312) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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