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[KD Validated] ABCA6 Rabbit pAb (A21243)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] ABCA6 Rabbit pAb (A21243)

Western blot analysis of extracts of various cell lines, using ABCA6 antibody (A21243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] ABCA6 Rabbit pAb (A21243)

Western blot analysis of extracts from wild type(WT) and ABCA6 knockdown (KD) 293T cells, using ABCA6 antibody (A21243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name[KD Validated] ABCA6 Rabbit pAb
Catalog No.A21243
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This gene is clustered among 4 other ABC1 family members on 17q24 and may play a role in macrophage lipid homeostasis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1518-1617 of human ABCA6 (NP_525023.2).
SequenceSQVTLVHTEILKLFPQAAGQERYSSLLTYKLPVADVYPLSQTFHKLEAVKHNFNLEEYSLSQCTLEKVFLELSKEQEVGNFDEEIDTTMRWKLLPHSDEP
Gene ID
Swiss Prot
SynonymsEST155051
Calculated MW184kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, RAW264.7, Mouse liver, Rat liver
Cellular locationMembrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] ABCA6 Rabbit pAb (A21243)}

Western blot - [KD Validated] ABCA6 Rabbit pAb (A21243)

Western blot analysis of extracts of various cell lines, using ABCA6 antibody (A21243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] ABCA6 Rabbit pAb (A21243)}

Western blot - [KD Validated] ABCA6 Rabbit pAb (A21243)

Western blot analysis of extracts from wild type(WT) and ABCA6 knockdown (KD) 293T cells, using ABCA6 antibody (A21243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)