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[KD Validated] DCK Rabbit mAb (A24071)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] DCK Rabbit mAb (A24071)

Western blot analysis of lysates from Daudi cells using [KD Validated] DCK Rabbit mAb(A24071) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:60s.

ABclonal:Western blot - [KD Validated] DCK Rabbit mAb (A24071)

Western blot analysis of lysates from wild type (WT) and DCK knockdown (KD) 293F cells using [KD Validated] DCK Rabbit mAb (A24071) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 60s.

ABclonal:Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)

Immunohistochemistry analysis of DCK in paraffin-embedded human tonsil tissue using [KD Validated] DCK Rabbit mAb (A24071) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)

Immunohistochemistry analysis of DCK in paraffin-embedded mouse spleen tissue using [KD Validated] DCK Rabbit mAb (A24071) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)

Immunohistochemistry analysis of DCK in paraffin-embedded rat spleen tissue using [KD Validated] DCK Rabbit mAb (A24071) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name[KD Validated] DCK Rabbit mAb
Catalog No.A24071
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65397
Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DCK (NP_000779.1).
SequenceMATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL
Gene ID
Swiss Prot
SynonymsDCK; [KD Validated] DCK
Calculated MW31kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesDaudi, 293F
Cellular locationNucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KD Validated] DCK Rabbit mAb (A24071)}

    Western blot - [KD Validated] DCK Rabbit mAb (A24071)

    Western blot analysis of lysates from Daudi cells using [KD Validated] DCK Rabbit mAb(A24071) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime:60s.
    ABclonal:Western blot - [KD Validated] DCK Rabbit mAb (A24071)}

    Western blot - [KD Validated] DCK Rabbit mAb (A24071)

    Western blot analysis of lysates from wild type (WT) and DCK knockdown (KD) 293F cells using [KD Validated] DCK Rabbit mAb (A24071) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime: 60s.
    ABclonal:Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)}

    Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)

    Immunohistochemistry analysis of DCK in paraffin-embedded human tonsil tissue using [KD Validated] DCK Rabbit mAb (A24071) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)}

    Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)

    Immunohistochemistry analysis of DCK in paraffin-embedded mouse spleen tissue using [KD Validated] DCK Rabbit mAb (A24071) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)}

    Immunohistochemistry - [KD Validated] DCK Rabbit mAb (A24071)

    Immunohistochemistry analysis of DCK in paraffin-embedded rat spleen tissue using [KD Validated] DCK Rabbit mAb (A24071) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.