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[KD Validated] OAT Rabbit mAb (A27191)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] OAT Rabbit mAb (A27191)

Western blot analysis of various lysates using [KD Validated] OAT Rabbit mAb (A27191) at 1:11000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - [KD Validated] OAT Rabbit mAb (A27191)

Western blot analysis of lysates from wild type (WT) and OAT knockdown (KD) 293T cells using [KD Validated] OAT Rabbit mAb (A27191) at 1:11000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)

Confocal imaging of Hep G2 cells using [KD Validated] OAT Rabbit mAb (A27191, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)

Confocal imaging of A-431 cells using [KD Validated] OAT Rabbit mAb (A27191, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)

Confocal imaging of C6 cells using [KD Validated] OAT Rabbit mAb (A27191, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KD Validated] OAT Rabbit mAb
Catalog No.A27191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC72769
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).
SequenceVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF
Gene ID
Swiss Prot
SynonymsOKT; GACR; HOGA; OATASE
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:11000 - 1:44000
  • IHC-P 1:1000 - 1:10000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHep G2, Mouse liver, Mouse kidney, Rat liver, 293T
Cellular locationMitochondrion matrix.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] OAT Rabbit mAb (A27191)}

Western blot - [KD Validated] OAT Rabbit mAb (A27191)

Western blot analysis of various lysates using [KD Validated] OAT Rabbit mAb (A27191) at 1:11000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - [KD Validated] OAT Rabbit mAb (A27191)}

Western blot - [KD Validated] OAT Rabbit mAb (A27191)

Western blot analysis of lysates from wild type (WT) and OAT knockdown (KD) 293T cells using [KD Validated] OAT Rabbit mAb (A27191) at 1:11000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)}

Immunohistochemistry - [KD Validated] OAT Rabbit mAb (A27191)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] OAT Rabbit mAb (A27191) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)}

Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)

Confocal imaging of Hep G2 cells using [KD Validated] OAT Rabbit mAb (A27191, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)}

Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)

Confocal imaging of A-431 cells using [KD Validated] OAT Rabbit mAb (A27191, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)}

Immunofluorescence - [KD Validated] OAT Rabbit mAb (A27191)

Confocal imaging of C6 cells using [KD Validated] OAT Rabbit mAb (A27191, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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