Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of lysates from Mouse thymus using [KD Validated] SNRNP70 Rabbit mAb (A24073) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and SNRNP70 knockdown (KD) 293T cells using [KD Validated] SNRNP70 Rabbit mAb (A24073) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020)
Exposure time: 90s.
| Product name | [KD Validated] SNRNP70 Rabbit mAb |
|---|---|
| Catalog No. | A24073 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC64486 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 201-300 of SNRNP70 (NP_003080.2). |
|---|---|
| Sequence | RGGADVNIRHSGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRERSKDKDRDRKRRSSRSRERAR |
| Gene ID | |
| Swiss Prot | |
| Synonyms | RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K |
| Calculated MW | 52kDa |
| Observed MW | 63kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T, Mouse thymus |
| Cellular location | Nucleus, Nucleus speckle, nucleoplasm. |
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* For research use only. Not for therapeutic or diagnostic purposes.
![ABclonal:Western blot - [KD Validated] SNRNP70 Rabbit mAb (A24073)](https://img.abclonal.com/abclonal-manage/Catalog/A24073/A24073_1.jpg?t=1727917528 "ABclonal:Western blot - [KD Validated] SNRNP70 Rabbit mAb (A24073)")
![ABclonal:Western blot - [KD Validated] SNRNP70 Rabbit mAb (A24073)](https://img.abclonal.com/abclonal-manage/Catalog/A24073/A24073_2.jpg?t=1727917528 "ABclonal:Western blot - [KD Validated] SNRNP70 Rabbit mAb (A24073)")
