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[KD Validated] STAT2 Rabbit mAb (A3588)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)

Western blot analysis of lysates from Rat liver, using [KD Validated] STAT2 Rabbit mAb (A3588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)

Western blot analysis of various lysates using [KD Validated] STAT2 Rabbit mAb (A3588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)

Western blot analysis of lysates from wild type (WT) and STAT2 knockdown (KD) 293T cells, using [KD Validated] STAT2 Rabbit mAb (A3588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name[KD Validated] STAT2 Rabbit mAb
Catalog No.A3588
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51796
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. The protein mediates innate antiviral activity. Mutations in this gene result in Immunodeficiency 44.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 550-706 of human STAT2 (NP_005410.1).
SequenceKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDEL
Gene ID
Swiss Prot
SynonymsP113; IMD44; ISGF-3; PTORCH3; STAT113; [KD Validated] STAT2
Calculated MW98kDa
Observed MW97kDa/113kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, A-549, A-431, Rat liver
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus, Homo sapiens, Mus musculus)

IF(Mus musculus)

IP(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)}

Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)

Western blot analysis of lysates from Rat liver, using [KD Validated] STAT2 Rabbit mAb (A3588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)}

Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)

Western blot analysis of various lysates using [KD Validated] STAT2 Rabbit mAb (A3588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)}

Western blot - [KD Validated] STAT2 Rabbit mAb (A3588)

Western blot analysis of lysates from wild type (WT) and STAT2 knockdown (KD) 293T cells, using [KD Validated] STAT2 Rabbit mAb (A3588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)