제품 > 항체 > 단일클론항체(mAb)

[KD Validated] Smad2 Rabbit mAb (A19114)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells using [KD Validated] Smad2 Rabbit mAb (A19114) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of various lysates using [KD Validated] Smad2 Rabbit mAb (A19114) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from Rat lung using [KD Validated] Smad2 Rabbit mAb (A19114) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - [KD Validated] Smad2 Rabbit mAb (A19114)

Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] Smad2 Rabbit mAb (A19114) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal imaging of HeLa cells using [KD Validated] Smad2 Rabbit mAb (A19114, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KD Validated] Smad2 Rabbit mAb (A19114)

Immunoprecipitation of Smad2 from 300 µg extracts of HeLa cells was performed using 3 µg of Smad2 antibody (A19114). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Smad2 antibody (A19114) at a dilution of 1:1000.

Overview

Product name	[KD Validated] Smad2 Rabbit mAb
Catalog No.	A19114
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0343

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (Q15796).
Sequence	MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTG
Gene ID
Swiss Prot
Synonyms	JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; [KD Validated] Smad2
Calculated MW	52kDa
Observed MW	58kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:100 - 1:400 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, HT-29, Jurkat, NIH/3T3, Rat lung
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Oryctolagus cuniculus, Homo sapiens, Rattus norvegicus) IP(Homo sapiens) IHC(Mus musculus) IHC(Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] Smad2 Rabbit mAb images

Western blot - [KD Validated] Smad2 Rabbit mAb (A19114)

Immunofluorescence - [KD Validated] Smad2 Rabbit mAb (A19114)

Immunoprecipitation - [KD Validated] Smad2 Rabbit mAb (A19114)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Mus musculus

PMID:

32783983

Title:

Baihe Wuyao decoction ameliorates CCl4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Gene

Application:

IF:

3.91

Species:

Oryctolagus cuniculus

PMID:

33333220

Title:

Characterization and functional analysis of SMAD2 regulation in hair follicle cycle in Angora rabbits

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

The Journal of clinical investigation

Application:

IF:

19.45

Species:

Mus musculus

PMID:

35085106

Title:

Smad3 methylation by EZH2 promotes its activation and tumor metastasis

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Advanced healthcare materials

Application:

IF:

11.09

Species:

Mus musculus

PMID:

36603196

Title:

Antifibrotic Effects of Tetrahedral Framework Nucleic Acids by Inhibiting Macrophage Polarization and Macrophage‐myofibroblast Transition in Bladder Remodeling

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

BMC microbiology

Application:

IF:

4.46

Species:

Mus musculus

PMID:

36949381

Title:

Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice - PMC

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

WB, IP

IF:

5.83

Species:

Homo sapiens

PMID:

36468780

Title:

VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Homo sapiens

PMID:

38362150

Title:

Alpha-hederin reprograms multi-miRNAs activity and overcome small extracellular vesicles-mediated paclitaxel resistance in NSCLC - PMC

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Homo sapiens

PMID:

38071381

Title:

PNO1 promotes the progression of osteosarcoma via TGF- and YAP/TAZ pathway - PMC

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Oncogene

Application:

IF:

8.75

Species:

Homo sapiens

PMID:

36991117

Title:

PRMT5 methylating Smad4 activates TGF- signaling and promotes colorectal cancer metastasis

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

7.9

Species:

Mus musculus

PMID:

38452697

Title:

Paris polyphylla ethanol extract and polyphyllin I ameliorate adenomyosis by inhibiting epithelialmesenchymal transition

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

5.7

Species:

Homo sapiens,Mus musculus

PMID:

38424494

Title:

USF1 transcriptionally activates USP14 to drive atherosclerosis by promoting EndMT through NLRC5/Smad2/3 axis - PMC

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Journal of genetics and genomics = Yi chuan xue bao

Application:

IF:

5.9

Species:

Homo sapiens

PMID:

38461943

Title:

Weakened tanning ability is an important mechanism for evolutionary skin lightening in East Asians

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Life sciences

Application:

IF:

6.78

Species:

Rattus norvegicus

PMID:

35378138

Title:

Biochanin A alleviates unilateral ureteral obstruction-induced renal interstitial fibrosis and inflammation by inhibiting the TGF-1/Smad2/3 and NF-kB/NLRP3 signaling axis in mice

References for [KD Validated] Smad2 Rabbit mAb (A19114)

Product:

[KD Validated] Smad2 Rabbit mAb

Journal:

Molecular carcinogenesis

Application:

WB, IHC

IF:

5.13

Species:

Mus musculus

PMID: