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[KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Western blot analysis of lysates from wild type (WT) and TKT knockdown (KD) HeLa cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Western blot - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Western blot analysis of various lysates using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of HeLa cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of Hep G2 cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of A549 cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of NIH/3T3 cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KD Validated] TKT/Transketolase Rabbit mAb
Catalog No.A27144
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC69667
This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 281-380 of human TKT/Transketolase (NP_001055.1).
SequenceKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRN
Gene ID
Swiss Prot
SynonymsTK; TKT1; SDDHD; HEL107; HEL-S-48
Calculated MW68kDa
Observed MW68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:6500 - 1:26000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse thymus, Mouse kindey, HeLa
Cellular locationcytosol, extracellular exosome, nuclear body, nucleoplasm, peroxisome.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Western blot - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Western blot analysis of lysates from wild type (WT) and TKT knockdown (KD) HeLa cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Western blot - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Western blot - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Western blot analysis of various lysates using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunohistochemistry - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using [KD Validated] TKT/Transketolase Rabbit mAb (A27144) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of HeLa cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of Hep G2 cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of A549 cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)}

Immunofluorescence - [KD Validated] TKT/Transketolase Rabbit mAb (A27144)

Confocal imaging of NIH/3T3 cells using [KD Validated] TKT/Transketolase Rabbit mAb (A27144, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)