제품 > 항체 > 다중클론항체(pAb)

KIF20B Rabbit pAb (A15360)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIF20B Rabbit pAb (A15360)

Western blot analysis of various lysates using KIF20B Rabbit pAb (A15360) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameKIF20B Rabbit pAb
Catalog No.A15360
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables several functions, including WW domain binding activity; plus-end-directed microtubule motor activity; and protein homodimerization activity. Involved in positive regulation of cell population proliferation and positive regulation of cytokinesis. Located in several cellular components, including microtubule cytoskeleton; midbody; and nuclear lumen.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1200-1500 of human KIF20B (NP_057279.2).
SequenceKHLLQLKEEEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRSNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKYAEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSN
Gene ID
Swiss Prot
SynonymsCT90; MPP1; KRMP1; MPP-1; MPHOSPH1; KIF20B
Calculated MW211kDa
Observed MW210kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-251MG, OVCAR3, 293T, HT-29, NIH/3T3, Mouse brain, Rat brain
Cellular locationCell projection, Cytoplasm, Midbody, Nucleus, axon, centrosome, cytoskeleton, growth cone, microtubule organizing center, nucleolus, nucleoplasm, spindle, spindle pole
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - KIF20B Rabbit pAb (A15360)}

Western blot - KIF20B Rabbit pAb (A15360)

Western blot analysis of various lysates using KIF20B Rabbit pAb (A15360) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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