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KIF4A Rabbit mAb (A9080)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIF4A Rabbit mAb (A9080)

Western blot analysis of various lysates using KIF4A Rabbit mAb (A9080) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - KIF4A Rabbit mAb (A9080)

Immunohistochemistry analysis of paraffin-embedded Rat testis using KIF4A Rabbit mAb (A9080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KIF4A Rabbit mAb (A9080)

Immunohistochemistry analysis of paraffin-embedded Human colon using KIF4A Rabbit mAb (A9080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KIF4A Rabbit mAb (A9080)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using KIF4A Rabbit mAb (A9080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameKIF4A Rabbit mAb
Catalog No.A9080
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1405
This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIF4A (O95239).
SequenceMKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGG
Gene ID
Swiss Prot
SynonymsKIF4; KIF4G1; MRX100; XLID100; KIF4A
Calculated MW140kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, A549
Cellular locationAxon cytoplasm, Cytoplasm, Cytosol, Nuclear matrix, Nucleoplasm, Spindle microtubule

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ABclonal:Western blot - KIF4A Rabbit mAb (A9080)}

Western blot - KIF4A Rabbit mAb (A9080)

Western blot analysis of various lysates using KIF4A Rabbit mAb (A9080) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - KIF4A Rabbit mAb (A9080)}

Immunohistochemistry - KIF4A Rabbit mAb (A9080)

Immunohistochemistry analysis of paraffin-embedded Rat testis using KIF4A Rabbit mAb (A9080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - KIF4A Rabbit mAb (A9080)}

Immunohistochemistry - KIF4A Rabbit mAb (A9080)

Immunohistochemistry analysis of paraffin-embedded Human colon using KIF4A Rabbit mAb (A9080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - KIF4A Rabbit mAb (A9080)}

Immunohistochemistry - KIF4A Rabbit mAb (A9080)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using KIF4A Rabbit mAb (A9080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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