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KIF5A Rabbit pAb (A3303)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIF5A Rabbit pAb (A3303)

Western blot analysis of various lysates using KIF5A Rabbit pAb (A3303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - KIF5A Rabbit pAb (A3303)

Immunofluorescence analysis of PC-12 cells using KIF5A Rabbit pAb (A3303) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KIF5A Rabbit pAb (A3303)

Immunofluorescence analysis of SH-SY5Y cells using KIF5A Rabbit pAb (A3303) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKIF5A Rabbit pAb
Catalog No.A3303
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2).
SequenceTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS
Gene ID
Swiss Prot
SynonymsNKHC; ALS25; MY050; NEIMY; SPG10; D12S1889; KIF5A
Calculated MW117kDa
Observed MW129kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:3000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCytoplasm, cytoskeleton, perinuclear region
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - KIF5A Rabbit pAb (A3303)}

Western blot - KIF5A Rabbit pAb (A3303)

Western blot analysis of various lysates using KIF5A Rabbit pAb (A3303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - KIF5A Rabbit pAb (A3303)}

Immunofluorescence - KIF5A Rabbit pAb (A3303)

Immunofluorescence analysis of PC-12 cells using KIF5A Rabbit pAb (A3303) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KIF5A Rabbit pAb (A3303)}

Immunofluorescence - KIF5A Rabbit pAb (A3303)

Immunofluorescence analysis of SH-SY5Y cells using KIF5A Rabbit pAb (A3303) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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