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KIFAP3 Rabbit mAb (A5216)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIFAP3 Rabbit mAb (A5216)

Western blot analysis of extracts of various cell lines, using KIFAP3 Rabbit mAb (A5216) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - KIFAP3 Rabbit mAb (A5216)

Immunofluorescence analysis of NIH-3T3 cells using KIFAP3 Rabbit mAb (A5216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KIFAP3 Rabbit mAb (A5216)

Immunofluorescence analysis of U-2 OS cells using KIFAP3 Rabbit mAb (A5216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameKIFAP3 Rabbit mAb
Catalog No.A5216
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1211
The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 675-792 of human KIFAP3 (Q92845).
SequenceKFRWHNSQWLEMVESRQMDESEQYLYGDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGILGRPATAYGFRPDEPYYYGYGS
Gene ID
Swiss Prot
SynonymsFLA3; KAP3; SMAP; KAP-1; KAP-3; Smg-GDS; dJ190I16.1
Calculated MW91kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Mouse brain, Rat brain
Cellular locationaxoneme, centrosome, ciliary basal body, condensed nuclear chromosome, cytosol, endoplasmic reticulum, Golgi apparatus, microtubule cytoskeleton, photoreceptor inner segment, photoreceptor outer segment

Documents

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    ABclonal:Western blot - KIFAP3 Rabbit mAb (A5216)}

    Western blot - KIFAP3 Rabbit mAb (A5216)

    Western blot analysis of extracts of various cell lines, using KIFAP3 Rabbit mAb (A5216) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - KIFAP3 Rabbit mAb (A5216)}

    Immunofluorescence - KIFAP3 Rabbit mAb (A5216)

    Immunofluorescence analysis of NIH-3T3 cells using KIFAP3 Rabbit mAb (A5216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - KIFAP3 Rabbit mAb (A5216)}

    Immunofluorescence - KIFAP3 Rabbit mAb (A5216)

    Immunofluorescence analysis of U-2 OS cells using KIFAP3 Rabbit mAb (A5216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)