제품 > 항체 > 다중클론항체(pAb)

KIFC1 Rabbit pAb (A3304)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIFC1 Rabbit pAb (A3304)

Western blot analysis of various lysates using KIFC1 Rabbit pAb (A3304) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - KIFC1 Rabbit pAb (A3304)

Immunohistochemistry analysis of paraffin-embedded rat testis using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KIFC1 Rabbit pAb (A3304)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KIFC1 Rabbit pAb (A3304)

Immunohistochemistry analysis of paraffin-embedded mouse testis using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - KIFC1 Rabbit pAb (A3304)

Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKIFC1 Rabbit pAb
Catalog No.A3304
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable microtubule binding activity and minus-end-directed microtubule motor activity. Involved in mitotic metaphase plate congression and mitotic spindle assembly. Located in membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2).
SequenceMDPQRSPLLEVKGNIELKRPLIKAPSQLPLSGSRLKRRPDQMEDGLEPEKKRTRGLGATTKITTSHPRVPSLTTVPQTQGQTTAQKVSKKTGPRCSTAIATGLKNQKPVPAVPVQKSGTSGVPPMAGGKKPSKRPAWDLKGQLCDLNAELKRCRERTQTLDQENQQLQDQLRDAQQQVKALGTERTTLEGHLAKVQAQAEQGQQELKNLRACVLELEERLSTQEGLVQELQKKQVELQEERRGLMSQLEE
Gene ID
Swiss Prot
SynonymsHSET; KNSL2; KIFC1
Calculated MW74kDa
Observed MW74kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, HT-29, HeLa, A-549
Cellular locationCytoplasm, Early endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

IHC(Homo sapiens)

IF(Homo sapiens)

WB(Homo sapiens, Rattus norvegicus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - KIFC1 Rabbit pAb (A3304)}

Western blot - KIFC1 Rabbit pAb (A3304)

Western blot analysis of various lysates using KIFC1 Rabbit pAb (A3304) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - KIFC1 Rabbit pAb (A3304)}

Immunohistochemistry - KIFC1 Rabbit pAb (A3304)

Immunohistochemistry analysis of paraffin-embedded rat testis using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - KIFC1 Rabbit pAb (A3304)}

Immunohistochemistry - KIFC1 Rabbit pAb (A3304)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - KIFC1 Rabbit pAb (A3304)}

Immunohistochemistry - KIFC1 Rabbit pAb (A3304)

Immunohistochemistry analysis of paraffin-embedded mouse testis using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - KIFC1 Rabbit pAb (A3304)}

Immunofluorescence - KIFC1 Rabbit pAb (A3304)

Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit pAb (A3304) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3304? Please let us know so that we can cite the reference in this datasheet.