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[KO Validated] CHD1 Rabbit pAb (A7883)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] CHD1 Rabbit pAb (A7883)

Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (A7883) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] CHD1 Rabbit pAb (A7883)

Western blot analysis of lysates from Jurkat cells, using [KO Validated] CHD1 Rabbit pAb (A7883) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] CHD1 Rabbit pAb (A7883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using [KO Validated] CHD1 Rabbit pAb (A7883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)

Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] CHD1 Rabbit pAb (A7883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name[KO Validated] CHD1 Rabbit pAb
Catalog No.A7883
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
SequenceDREKHRKLDDHRSRDHRSNLEGSLKDRSHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQMDHRASSSGPRSPLDQRSPYGSRSPFEHSVEHKSTPEHTWSSRKT
Gene ID
Swiss Prot
SynonymsCHD-1; PILBOS; D1
Calculated MW197kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] CHD1 Rabbit pAb (A7883)}

Western blot - [KO Validated] CHD1 Rabbit pAb (A7883)

Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (A7883) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CHD1 Rabbit pAb (A7883)}

Western blot - [KO Validated] CHD1 Rabbit pAb (A7883)

Western blot analysis of lysates from Jurkat cells, using [KO Validated] CHD1 Rabbit pAb (A7883) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)}

Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] CHD1 Rabbit pAb (A7883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)}

Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using [KO Validated] CHD1 Rabbit pAb (A7883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)}

Immunohistochemistry - [KO Validated] CHD1 Rabbit pAb (A7883)

Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] CHD1 Rabbit pAb (A7883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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