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[KO Validated] CSNK1E Rabbit pAb (A1796)

KO/KDValidated

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] CSNK1E Rabbit pAb (A1796)

Western blot analysis of various lysates using [KO Validated] CSNK1E Rabbit pAb (A1796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] CSNK1E Rabbit pAb (A1796)

Western blot analysis of lysates from wild type (WT) and CSNK1E knockout (KO) HeLa cells, using [KO Validated] CSNK1E Rabbit pAb (A1796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - [KO Validated] CSNK1E Rabbit pAb (A1796)

Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit pAb (A1796). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] CSNK1E Rabbit pAb
Catalog No.A1796
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
SequenceEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK
Gene ID
Swiss Prot
SynonymsCKIe; HCKIE; CKIepsilon; 1E
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] CSNK1E Rabbit pAb (A1796)}

Western blot - [KO Validated] CSNK1E Rabbit pAb (A1796)

Western blot analysis of various lysates using [KO Validated] CSNK1E Rabbit pAb (A1796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CSNK1E Rabbit pAb (A1796)}

Western blot - [KO Validated] CSNK1E Rabbit pAb (A1796)

Western blot analysis of lysates from wild type (WT) and CSNK1E knockout (KO) HeLa cells, using [KO Validated] CSNK1E Rabbit pAb (A1796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] CSNK1E Rabbit pAb (A1796)}

Immunofluorescence - [KO Validated] CSNK1E Rabbit pAb (A1796)

Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit pAb (A1796). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1796? Please let us know so that we can cite the reference in this datasheet.