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[KO Validated] MEK7 Rabbit mAb (A12950)

KO/KDValidated

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] MEK7 Rabbit mAb (A12950)

Western blot analysis of lysates from wild type(WT) and MEK7 knockout (KO) 293T(KO) cells, using [KO Validated] MEK7 Rabbit mAb (A12950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name[KO Validated] MEK7 Rabbit mAb
Catalog No.A12950
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0712
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 320-419 of human MEK7 (O14733).
SequenceFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQPHLPFFR
Gene ID
Swiss Prot
SynonymsMEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4; K7
Calculated MW47kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] MEK7 Rabbit mAb (A12950)}

Western blot - [KO Validated] MEK7 Rabbit mAb (A12950)

Western blot analysis of lysates from wild type(WT) and MEK7 knockout (KO) 293T(KO) cells, using [KO Validated] MEK7 Rabbit mAb (A12950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12950? Please let us know so that we can cite the reference in this datasheet.