Western blot - [KO Validated] MSH6 Rabbit pAb (A21445)
Western blot analysis of various lysates using MSH6 Rabbit pAb (A21445) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot - [KO Validated] MSH6 Rabbit pAb (A21445)
Western blot analysis of various lysates using [KO Validated] MSH6 Rabbit pAb (A21445) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 10 s.
Western blot - [KO Validated] MSH6 Rabbit pAb (A21445)
Western blot analysis of lysates from wild type (WT) and MSH6 knockout (KO) 293T cells using [KO Validated] MSH6 Rabbit pAb (A21445) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 10 s.
Immunohistochemistry - [KO Validated] MSH6 Rabbit pAb (A21445)
Immunohistochemistry analysis of paraffin-embedded Human colon cancer (loss of MSH6 expression) using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KO Validated] MSH6 Rabbit pAb (A21445)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KO Validated] MSH6 Rabbit pAb (A21445)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - [KO Validated] MSH6 Rabbit pAb (A21445)
Immunofluorescence analysis of A-549 cells using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] MSH6 Rabbit pAb (A21445)
Immunofluorescence analysis of U2OS cells using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.