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[KO Validated] PSME3 Rabbit pAb (A18021)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] PSME3 Rabbit pAb (A18021)

Western blot analysis of various lysates using PSME3 Rabbit pAb (A18021) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] PSME3 Rabbit pAb (A18021)

Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit pAb (A18021) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunofluorescence analysis of NIH-3T3 cells using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] PSME3 Rabbit pAb
Catalog No.A18021
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-254 of human PSME3 (NP_005780.2).
SequenceEDYRRTVTEIDEKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNAETLY
Gene ID
Swiss Prot
SynonymsKi; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma; E3
Calculated MW30kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Paeonia)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] PSME3 Rabbit pAb (A18021)}

Western blot - [KO Validated] PSME3 Rabbit pAb (A18021)

Western blot analysis of various lysates using PSME3 Rabbit pAb (A18021) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] PSME3 Rabbit pAb (A18021)}

Western blot - [KO Validated] PSME3 Rabbit pAb (A18021)

Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit pAb (A18021) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)}

Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)}

Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)}

Immunohistochemistry - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] PSME3 Rabbit pAb (A18021)}

Immunofluorescence - [KO Validated] PSME3 Rabbit pAb (A18021)

Immunofluorescence analysis of NIH-3T3 cells using PSME3 Rabbit pAb (A18021) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)