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[KO Validated] SHP2 Rabbit pAb (A12486)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SHP2 Rabbit pAb (A12486)

Western blot analysis of lysates from wild type (WT) and SHP2 knockout (KO) 293T cells, using [KO Validated] SHP2 Rabbit pAb (A12486) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] SHP2 Rabbit pAb (A12486)

Western blot analysis of various lysates using [KO Validated] SHP2 Rabbit pAb (A12486) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - [KO Validated] SHP2 Rabbit pAb (A12486)

Immunofluorescence analysis of C6 cells using [KO Validated] SHP2 Rabbit pAb (A12486) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SHP2 Rabbit pAb (A12486)

Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg SHP2 antibody (A12486). Western blot was performed from the immunoprecipitate using SHP2 antibody (A12486) at a dilution of 1:2000.

Overview

Product name[KO Validated] SHP2 Rabbit pAb
Catalog No.A12486
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human SHP2 (NP_002825.3).
SequenceMTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCS
Gene ID
Swiss Prot
SynonymsCFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3; P2
Calculated MW68kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, Jurkat, A-431, MCF-7, Mouse brain, Rat brain, Rat lung
Cellular locationCytoplasm.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] SHP2 Rabbit pAb (A12486)}

Western blot - [KO Validated] SHP2 Rabbit pAb (A12486)

Western blot analysis of lysates from wild type (WT) and SHP2 knockout (KO) 293T cells, using [KO Validated] SHP2 Rabbit pAb (A12486) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] SHP2 Rabbit pAb (A12486)}

Western blot - [KO Validated] SHP2 Rabbit pAb (A12486)

Western blot analysis of various lysates using [KO Validated] SHP2 Rabbit pAb (A12486) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - [KO Validated] SHP2 Rabbit pAb (A12486)}

Immunofluorescence - [KO Validated] SHP2 Rabbit pAb (A12486)

Immunofluorescence analysis of C6 cells using [KO Validated] SHP2 Rabbit pAb (A12486) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SHP2 Rabbit pAb (A12486)}

Immunoprecipitation - [KO Validated] SHP2 Rabbit pAb (A12486)

Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg SHP2 antibody (A12486). Western blot was performed from the immunoprecipitate using SHP2 antibody (A12486) at a dilution of 1:2000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12486? Please let us know so that we can cite the reference in this datasheet.

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