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[KO Validated] SOD2 Rabbit mAb (A19576)

KO/KDValidated

Publications (14) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SOD2 Rabbit mAb (A19576)

Western blot analysis of various lysates, using SOD2 Rabbit mAb (A19576) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - [KO Validated] SOD2 Rabbit mAb (A19576)

Western blot analysis of lysates from wild type(WT) and SOD2 knockout (KO) 293T(KO) cells, using SOD2 Rabbit mAb (A19576) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using [KO Validated] SOD2 Rabbit mAb (A19576) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] SOD2 Rabbit mAb (A19576) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SOD2 Rabbit mAb (A19576) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] SOD2 Rabbit mAb (A19576)

Confocal imaging of paraffin-embedded Rat brain tissue using [KO Validated] SOD2 Rabbit mAb (A19576, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - [KO Validated] SOD2 Rabbit mAb (A19576)

Confocal imaging of paraffin-embedded Mouse brain tissue using [KO Validated] SOD2 Rabbit mAb (A19576, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product name[KO Validated] SOD2 Rabbit mAb
Catalog No.A19576
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0055
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SOD2 (P04179).
SequenceMLSRAVCGTSRQLAPVLGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSI
Gene ID
Swiss Prot
SynonymsGC1; IPOB; IPO-B; MNSOD; MVCD6; GClnc1; Mn-SOD; D2
Calculated MW25kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:8000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, NIH/3T3, RAW264.7, Rat brain, Rat heart
Cellular locationMitochondrion matrix.
Customer validation

WB(Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus)

IHC(Homo sapiens)

IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] SOD2 Rabbit mAb (A19576)}

Western blot - [KO Validated] SOD2 Rabbit mAb (A19576)

Western blot analysis of various lysates, using SOD2 Rabbit mAb (A19576) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - [KO Validated] SOD2 Rabbit mAb (A19576)}

Western blot - [KO Validated] SOD2 Rabbit mAb (A19576)

Western blot analysis of lysates from wild type(WT) and SOD2 knockout (KO) 293T(KO) cells, using SOD2 Rabbit mAb (A19576) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)}

Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using [KO Validated] SOD2 Rabbit mAb (A19576) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)}

Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] SOD2 Rabbit mAb (A19576) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)}

Immunohistochemistry - [KO Validated] SOD2 Rabbit mAb (A19576)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SOD2 Rabbit mAb (A19576) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] SOD2 Rabbit mAb (A19576)}

Immunofluorescence - [KO Validated] SOD2 Rabbit mAb (A19576)

Confocal imaging of paraffin-embedded Rat brain tissue using [KO Validated] SOD2 Rabbit mAb (A19576, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - [KO Validated] SOD2 Rabbit mAb (A19576)}

Immunofluorescence - [KO Validated] SOD2 Rabbit mAb (A19576)

Confocal imaging of paraffin-embedded Mouse brain tissue using [KO Validated] SOD2 Rabbit mAb (A19576, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19576? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

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A1340

ELISAWBIHC-PIF/ICC

Reactivity:Human, Mouse, Rat

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A21805

WBIHC-PIF/ICCELISA

Reactivity:Human,Mouse,Rat

SOD2 Rabbit pAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)