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[KO Validated] UBE2S Rabbit pAb (A18064)

KO/KDValidated

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] UBE2S Rabbit pAb (A18064)

Western blot analysis of various lysates using UBE2S Rabbit pAb (A18064) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] UBE2S Rabbit pAb (A18064)

Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit pAb (A18064) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name[KO Validated] UBE2S Rabbit pAb
Catalog No.A18064
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).
SequenceHPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTEASSTDPGAPGGPGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRR
Gene ID
Swiss Prot
SynonymsEPF5; E2EPF; E2-EPF; 2S
Calculated MW24kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationcytosol, nucleoplasm, nucleus.
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] UBE2S Rabbit pAb (A18064)}

Western blot - [KO Validated] UBE2S Rabbit pAb (A18064)

Western blot analysis of various lysates using UBE2S Rabbit pAb (A18064) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] UBE2S Rabbit pAb (A18064)}

Western blot - [KO Validated] UBE2S Rabbit pAb (A18064)

Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit pAb (A18064) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18064? Please let us know so that we can cite the reference in this datasheet.