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Kaiso/ZBTB33 Rabbit pAb (A12900)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Kaiso/ZBTB33 Rabbit pAb (A12900)

Western blot analysis of various lysates using Kaiso/ZBTB33 Rabbit pAb (A12900) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Chromatin Immunoprecipitation - Kaiso/ZBTB33 Rabbit pAb (A12900)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso/ZBTB33 antibody (A12900) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameKaiso/ZBTB33 Rabbit pAb
Catalog No.A12900
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human Kaiso/Kaiso/ZBTB33 (NP_006768.1).
SequencePCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSFINYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY
Gene ID
Swiss Prot
SynonymsZNF348; ZNF-kaiso; Kaiso/ZBTB33
Calculated MW74kDa
Observed MW74-90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Mouse kidney, Mouse skeletal muscle
Cellular locationCytoplasm, Nucleus.
Customer validation

ChIP(Mus musculus)

Documents

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    ABclonal:Western blot - Kaiso/ZBTB33 Rabbit pAb (A12900)}

    Western blot - Kaiso/ZBTB33 Rabbit pAb (A12900)

    Western blot analysis of various lysates using Kaiso/ZBTB33 Rabbit pAb (A12900) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Chromatin Immunoprecipitation - Kaiso/ZBTB33 Rabbit pAb (A12900)}

    Chromatin Immunoprecipitation - Kaiso/ZBTB33 Rabbit pAb (A12900)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso/ZBTB33 antibody (A12900) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12900? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)