제품 > 항체 > 다중클론항체(pAb)

LAMTOR1 Rabbit pAb (A21557)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using LAMTOR1 Rabbit pAb (A21557) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - LAMTOR1 Rabbit pAb (A21557)

Confocal immunofluorescence analysis of U2OS cells using LAMTOR1 Rabbit pAb (A21557) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	LAMTOR1 Rabbit pAb
Catalog No.	A21557
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables GTPase binding activity. Contributes to guanyl-nucleotide exchange factor activity and molecular adaptor activity. Involved in several processes, including cholesterol homeostasis; positive regulation of TOR signaling; and regulation of cholesterol transport. Located in lysosome. Part of Ragulator complex.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1).
Sequence	MGCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLASEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP
Gene ID
Swiss Prot
Synonyms	p18; PDRO; C11orf59; p27RF-Rho; Ragulator1; LAMTOR1
Calculated MW	18kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	B-cell, HeLa, Jurkat, U-87 MG, Mouse brain
Cellular location	Cell membrane, Cytoplasmic side, Late endosome membrane, Lipid-anchor, Lysosome membrane
Customer validation	WB(Gallus gallus)