제품 > 항체 > 다중클론항체(pAb)

LARP4 Rabbit pAb (A5108)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of Rat kidney, using LARP4 antibody (A5108) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - LARP4 Rabbit pAb (A5108)

Immunofluorescence analysis of NIH/3T3 cells using LARP4 Rabbit pAb (A5108) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	LARP4 Rabbit pAb
Catalog No.	A5108
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables mRNA 3'-UTR binding activity and poly(A) binding activity. Involved in cytoskeleton organization; positive regulation of translation; and regulation of cell morphogenesis. Located in cytosol. Colocalizes with cytoplasmic stress granule; cytosolic small ribosomal subunit; and polysome.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human LARP4 (NP_443111.4).
Sequence	MLLFVEQVASKGTGLNPNAKVWQEIAPGNTDATPVTHGTESSWHEIAATSGAHPEGNAELSEDICKEYEVMYSSSCETTRNTTGIEESTDGMILGPEDLSYQIYDVSGESNSAVSTEDLK
Gene ID
Swiss Prot
Synonyms	PP13296
Calculated MW	81kDa
Observed MW	81kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Rat kidney
Cellular location	cytoplasmic stress granule, cytosol
Customer validation	WB(Homo sapiens) IF(Homo sapiens)