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LC3A/LC3B Rabbit PolymAb® (A27200PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LC3A/LC3B Rabbit PolymAb® (A27200PM)

Western blot analysis of various lysates using LC3A/LC3B Rabbit PolymAb® (A27200PM) at 1:10000 dilution incubated at room temperature for 1.5 hours. Hela and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Western blot - LC3A/LC3B Rabbit PolymAb® (A27200PM)

Western blot analysis of lysates from NIH/3T3 cells using LC3A/LC3B Rabbit PolymAb® (A27200PM) at 1:10000 dilution incubated at room temperature for 1.5 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using LC3A/LC3B Rabbit PolymAb® (A27200PM) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using LC3A/LC3B Rabbit PolymAb® (A27200PM) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using LC3A/LC3B Rabbit PolymAb® (A27200PM) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - LC3A/LC3B Rabbit PolymAb® (A27200PM)

Confocal imaging of NIH/3T3 cells (treated with chloroquine) and NIH/3T3 cells (untreated) using LC3A/LC3B Rabbit PolymAb® (A27200PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameLC3A/LC3B Rabbit PolymAb®
Catalog No.A27200PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
ImmunogenA synthesized peptide derived from human LC3A/LC3B .
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsLC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; LC3A/B; LC3B
Calculated MW14kDa
Observed MW14kDa/16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:20000
  • IHC-P 1:2000 - 1:8000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, C6, NIH/3T3
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LC3A/LC3B Rabbit PolymAb® (A27200PM)}

    Western blot - LC3A/LC3B Rabbit PolymAb® (A27200PM)

    Western blot analysis of various lysates using LC3A/LC3B Rabbit PolymAb® (A27200PM) at 1:10000 dilution incubated at room temperature for 1.5 hours. Hela and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Western blot - LC3A/LC3B Rabbit PolymAb® (A27200PM)}

    Western blot - LC3A/LC3B Rabbit PolymAb® (A27200PM)

    Western blot analysis of lysates from NIH/3T3 cells using LC3A/LC3B Rabbit PolymAb® (A27200PM) at 1:10000 dilution incubated at room temperature for 1.5 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)}

    Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)

    Immunohistochemistry analysis of paraffin-embedded Human brain tissue using LC3A/LC3B Rabbit PolymAb® (A27200PM) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)}

    Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using LC3A/LC3B Rabbit PolymAb® (A27200PM) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)}

    Immunohistochemistry - LC3A/LC3B Rabbit PolymAb® (A27200PM)

    Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using LC3A/LC3B Rabbit PolymAb® (A27200PM) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - LC3A/LC3B Rabbit PolymAb® (A27200PM)}

    Immunofluorescence - LC3A/LC3B Rabbit PolymAb® (A27200PM)

    Confocal imaging of NIH/3T3 cells (treated with chloroquine) and NIH/3T3 cells (untreated) using LC3A/LC3B Rabbit PolymAb® (A27200PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.