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LILRB2/CD85d/ILT4 Rabbit pAb (A10135)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - LILRB2/CD85d/ILT4 Rabbit pAb (A10135)

Western blot analysis of extracts of various cell lines, using LILRB2/CD85d/ILT4 antibody (A10135) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameLILRB2/CD85d/ILT4 Rabbit pAb
Catalog No.A10135
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human LILRB2/CD85d/ILT4 (NP_005865.3).
SequenceQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVMAPGES
Gene ID
Swiss Prot
SynonymsILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
Calculated MW65kDa
Observed MW65kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver, Mouse kidney, Mouse blood cell, Rat liver, Rat kidney, Rat blood cell
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - LILRB2/CD85d/ILT4 Rabbit pAb (A10135)}

Western blot - LILRB2/CD85d/ILT4 Rabbit pAb (A10135)

Western blot analysis of extracts of various cell lines, using LILRB2/CD85d/ILT4 antibody (A10135) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

재조합 단백질 (7)

Secondary Antibodies (26)