제품 > 항체 > 다중클론항체(pAb)

LMBRD1 Rabbit pAb (A15866)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using LMBRD1 Rabbit pAb (A15866) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - LMBRD1 Rabbit pAb (A15866)

Immunofluorescence analysis of L929 cells using LMBRD1 Rabbit pAb (A15866) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	LMBRD1 Rabbit pAb
Catalog No.	A15866
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a lysosomal membrane protein that may be involved in the transport and metabolism of cobalamin. This protein also interacts with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 220-300 of human LMBRD1 (NP_060838.3).
Sequence	PLNLIKGTRSAAYERLENTEDIEEVEQHIQTIKSKSKDGRPLPARDKRALKQFEERLRTLKKRERHLEFIENSWWTKFCGA
Gene ID
Swiss Prot
Synonyms	NESI; LMBD1; MAHCF; C6orf209; LMBRD1
Calculated MW	61kDa
Observed MW	50kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-87MG, HeLa, Mouse brain, Mouse lung, Mouse spleen
Cellular location	Lysosome membrane, Multi-pass membrane protein
Customer validation	WB(Homo sapiens)